The invention discloses an antiseptic solution on-line monitoring system for a endoscope cleaning work station, and the antiseptic solution on-line monitoring system comprises a hardware system and a software system, the hardware system works under the management of the software system, the hardware system comprises a control module, a signal acquisition module, a communication module, a display control module and an alarm module, the signal acquisition module comprises a stock solution concentration signal acquisition unit, a pH signal acquisition unit and a temperature signal acquisition unit; the display control module comprises a touch screen; the alarm module comprises a alarm which is connected with a control module; the software system comprises a signal analysis module and a parameter setting module. According to the invention, automatic on-line monitoring of the cleaning fluid concentration is used for real time monitoring of the concentration changes of the cleaning antiseptic solution, thereby realizing intelligent monitoring management of the endoscope cleaning antiseptic solution, recording a present state of the effective concentration of the cleaning fluid, and the invention has the advantages of good accuracy, standard management of endoscope cleaning and disinfection without secondary pollution, and improved endoscope cleaning and disinfection quality.