The invention discloses a fluid driving type piezoelectric transducer based on a flexible ferroelectric film and a preparation method thereof. The fluid driving type piezoelectric transducer comprises a glass substrate and a micro-fluidic chip substrate, two phases of parallel P (VDF-TrFE) electro-spinning film strips are integrated on the glass substrate, a micro-channel and electrode grooves distributed at two sides of the micro-channel are manufactured on the micro-fluidic chip substrate, the micro-fluidic chip substrate is also provided with a sample inlet, a sample outlet, and silver paste inlets, the sample inlet and the sample outlet are communicated with two ends of the micro-channel, the silver paste inlets are communicated with the electrode grooves, the glass substrate and the micro-fluidic chip substrate are bonded, and the P (VDF-TrFE) electro-spinning film strips are positioned at the bottoms of the micro-channel and the electrode grooves. According to the fluid driving type piezoelectric transducer and the preparation method thereof, the manufacture is simple, the cost is low, large signal output under low-flow-velocity fluid drive is realized, the flow velocity of driving fluid can be tested, and the viscosity coefficient of the driving fluid can be tested.