利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105087627A 一种sll0659基因在合成集胞藻类胡萝卜素中的应用 有权

一种sll0659基因在合成集胞藻类胡萝卜素中的应用
摘要：
本发明涉及一种sll0659基因在合成集胞藻类胡萝卜素中的应用。该应用所涉及的sll0659基因，核苷酸序列如SEQ ID NO.1所示。本发明提供了一种在集胞藻PCC6803β-胡萝卜素合成中具有重要作用的集胞藻PCC6803中sll0659基因的应用，通过将集胞藻PCC6803中的sll0659基因敲除后，集胞藻PCC6803的叶黄素含量增加了22.3％，玉米黄素含量增加了31.3％，而海胆酮含量降低了34.3％，β-胡萝卜素百分含量则降低了1.3％。同时，HPLC检测结果表明，与野生型集胞藻PCC6803相比，sll0659基因敲除突变体中类胡萝卜素组分中有一个明显的峰，经与标品对照检测，确定其为α-胡萝卜素。
摘要（英）：

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

公开/授权文献
0/0