The invention discloses a light source apparatus for generation of comb-like spectrum for spectrometer spectrum calibration. The light source apparatus comprises a wide spectral light source, a collimation device, a Fabry-Perot etalon and a focusing mirror, wherein the wide spectral light source is used for emitting the light of continuum; the collimation device collimates setting of the wide spectral light source to collimate the emitted light which is coupled into the wide spectral light source; the Fabry-Perot etalon collimates setting of the collimation device so as to enable the light through parallel incidence from the collimation device to realize equal inclination interference, and filters a plurality of narrow-band lights with different wavelength in equifrequent space, wherein the plurality of narrow-band lights satisfy the transmission condition; and the focusing mirror collimates setting of the Fabry-Perot etalon so as to converge the narrow-band lights to a small emitting hole of the focusing mirror to form comb-like lights, wherein the spectrum components of the comb-like lights are narrow bands. The light source apparatus provides comb-like spectrum signals with high resolution and high density and can acquire the spectrum calibration data for a plurality of monochromatic channels in uniform space at a time; the characteristic wavelength number and the spray deposition uniformity of the comb-like spectral lines obtained by the comb-like spectrum light source system are better than the characteristic wavelength number and the spray deposition uniformity of the spectral lines of a traditional mercury argon light source; and the light source apparatus is specifically suitable for calibration of a raster spectrometer which employs polynomial fitting calibration.