The invention discloses a regulation information acceptance check method based on a replacing service. The regulation information acceptance check method is characterized by comprising the following steps: (1) setting a replacing service at a monitoring background to spacer layer equipment; (2) the spacer layer equipment transmits data change and quality change to a data communication gateway computer; (3) the data communication gateway computer transmits data to a regulation master station front-end computer; (4) the regulation master station front-end computer synchronously updates the received data to a regulation master station SCADA through a message bus; and (5) the value of the picture of the regulation master station SCADA and the value of the picture of the monitoring background are checked. The beneficial effect is that by using the replacing service of the monitoring background and checking the value of the picture of the regulation master station SCADA and the value of the picture of the monitoring background, regulation information acceptance check is realized, the problem that the traditional acceptance check method is low in efficiency is solved, the substation construction period is shortened, and the workload of the staff on a substation site is lightened.