The invention provides beef paste capable of relaxing the muscles and the tendons and strengthening the bones and a preparation method thereof. The beef paste is prepared from, by weight, 200-220 parts of beef, 200-220 parts of dry chillies, 200-220 parts of sweet flour sauce, 30-34 parts of crab meat, 8-10 parts of pearl barley, 15-18 parts of donkey bones, 0.7-0.9 part of glossy privet fruits, 0.5-0.6 part of hibiscus rosa-sinensis, 0.7-0.8 part of morinda officinalis, 0.6-0.8 part of oenothera biennis, 18-20 parts of salt, 10-12 parts of white granulated sugar, 3-4 parts of monosodium glutamate, an appropriate amount of papain and an appropriate amount of xanthan gum. The beef paste is fragrant and rich in taste, capable of promoting the appetite, tasty and refreshing and very delicious, multiple traditional Chinese medicines are added in the processing process, and therefore the effects of tonifying the livers and the kidneys, blacking hair, improving eyesight, clearing the lungs, dissipating dampness and strengthening the muscles, the tendons and the bones are achieved.