The invention relates to a multi-structure different-interval bionic surface combined cast-iron guide rail and a regeneration method thereof. First bionic coupling elements and second bionic coupling elements are made on mild wear areas and a severe wear area of the guide rail working face; the first bionic coupling elements are strip-shaped or net-shaped bionic coupling elements, and intervals of the first bionic coupling elements in the mild wear areas are larger than those of the first bionic coupling elements in the severe wear area; the second bionic coupling elements are pit-shaped coupling elements and are distributed among the first bionic coupling elements. According to the multi-structure different-interval bionic surface combined cast-iron guide rail and the regeneration method thereof, according to the rigidity or stress distribution situations of the areas with different wear degrees, two double-coupling-element bionic surfaces with changeable intervals are set, a multi-structure different-interval bionic surface combination with different anti-wear performances is formed, and the surface of which the rigidity or the stress is distributed evenly is obtained, so that the effect that the overall anti-wear performance of the surface is uniform and stable for a long time is achieved. In addition, by means of the interaction between the two coupling elements and a machine body, the wear resistance of the sliding guide rail is further improved.