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CN105112565A 一种检测伪狂犬病毒GE基因的LAMP检测方法 无效 - 驳回

一种检测伪狂犬病毒GE基因的LAMP检测方法
摘要：
为了快速、高效地检测猪伪狂犬病毒(PRV)，本发明采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)技术建立了一种针对伪狂犬病毒的可视、灵敏、快速、特异的检测方法。本发明提取的是临床样本DNA，更切合实际，只需要在65℃下恒温反应60min就能检测到结果，并且具有操作简便的优点，适合基层养殖场使用。
摘要（英）：

In order to quickly and efficiently detect pseudorabies viruses (PRV) of pigs, the invention establishes a visual, sensitive, quick and specific detection method aiming at the pseudorabies viruses by adopting a loop-mediated isothermal amplification (LAMP) technology. What extracted by the method provided by the invention is DNA of a clinical sample, and the method is more practical, can be used for detecting a result by only performing isothermal reaction for 60 minutes at 65 DEG C, has the advantage of simple and convenient operation and is suitable for use in grass-root farms.

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