The present invention provides a nuclear magnetic resonance logging instrument probe with multi-layered magnet and an antenna excitation method, the nuclear magnetic resonance logging instrument probe includes a probe skeleton, multiple magnet assemblies and a plurality of antennas; the probe skeleton is of a cylindrical shape, multiple magnet assemblies are distributed in the circumferential direction of the probe skeleton; the magnet assembly includes at least two layers of magnet arranged from top to bottom, the magnet is magnetized in a radial direction, two adjacent layers of magnet are magnetized in opposite directions; an antenna is arranged outside each magnet assembly, multiple antennas are independently fed. In the nuclear magnetic resonance logging instrument probe and antenna excitation method, through exciting different antennas, detection of stratum information at different azimuth angles is realized, which improves circumferential resolution of the probe, realize stratum detection in three dimensions along radius, axis and circumference.