The invention provides a method for biologically preventing and controlling leucaena glauca diffusion. The method includes the steps that multiple leucaena glauca seeds with leucaena glauca bean weevil eggs attached to the surfaces are made into an egg release seed bag, and then the egg release seed bag is placed in a low-temperature environment to be refrigerated to be used in the biological prevention and control release process; and/or multiple leucaena glauca seeds carrying leucaena glauca bean weevil larvae are made into a larva release seed bag, and then the larva release seed bag is placed in a low-temperature environment to be refrigerated to be used in the biological prevention and control release process; and/or multiple imagoes of first-filial-generation leucaena glauca bean weevil are made into an imago release bag, and then the imago release bag is placed in a low-temperature environment to be refrigerated to be used in the biological prevention and control release process. According to the method for biologically preventing and controlling leucaena glauca diffusion, the problems that the physical and mechanical felling is poor in effect and high in cost can be solved, cost is low, the acting effect is obvious, and the control effect can be continuously exerted.