The invention provides a Hucho taimen sperm activation solution and a preparation method thereof, and relates to the technical field of sperms of Hucho taimen species. The Hucho taimen sperm activation solution comprises the following raw materials: 40.64-81.27 mmol/L of glucose and 10.12-20.24 mmol/L of sodium chloride, wherein water temperature is 8-11 DEG C and osmotic pressure is 142.41 kPa to 284.81 kPa. The method comprises: weighing pure glucose and putting the pure glucose in a beaker; weighing analytically pure sodium chloride in the mass and putting the analytically pure sodium chloride in same beaker with the weighed glucose; adding deionized water or distilled water in half volume of the activation solution in the beaker, and stirring, transferring the dissolved solution in a volumetric flask whose capacity is the same with the prepared activation solution, pouring washed solution in the volumetric flask, and finally diluting with deionized water or distilled water to a scale mark, and shaking the volumetric flask. The activation solution and the preparation method solve problems of how to prolong movement time of Hucho taimen sperms and improve fertility rate.