A feeding box used for acanthoscelides macrophthalmus oviposition comprises a cuboid box body. The cuboid box body is composed of a lower box main body and an upper box cover covering the lower box main body. The internal space of the lower box main body and the internal space of the upper box cover form a whole. In the lower box main body, the bottom of the lower box main body is provided with a slope used for collecting seeds with eggs, and a foldable board matched with the slope and a movable board used for insect oviposition are hinged to the upper end of a groove through a rotary device. In the upper box cover, a slip rope device and a compartment are installed on the top face of the upper box cover, the slip rope device is used for feeding the acanthoscelides macrophthalmus with feed, and the compartment is matched with the slip rope device and used for adding feed. According to the feeding box used for acanthoscelides macrophthalmus oviposition and a use method of the feeding box, the problem that collection of the seeds with the eggs and feeding of acanthoscelides macrophthalmus adults are not easy is solved so that acanthoscelides macrophthalmus can successfully eat artificial feed; meanwhile, an oviposition place is formed, and large scale and standardization of the eggs used in experiment application are guaranteed.