The present disclosure relates to the field of display technology, and in particular, it discloses a display substrate assembly and a method of manufacturing the same, and a display apparatus. In order to solve a problem of inferior quality of a picture displayed in a conventional display apparatus caused due to non-uniformity of a thickness of a conventional display substrate assembly assembled. The present disclosure provides a display substrate assembly including a first substrate and a second substrate opposite to each other, the first substrate including a first region and a second region, and, a total thickness of functional layers within the first region being less than a total thickness of functional layers within the second region, of the first substrate. A thickness compensation layer is provided on at least one of the first substrate and the second substrate, a position of the thickness compensation layer corresponds to a position of the first region, and, a sum of thickness of a thickness of the thickness compensation layer and the total thickness of the functional layers within the first region equals to the total thickness of the functional layers within the second region. According to the present disclosure, the thickness compensation layer corresponding to the first region is provided between the first substrate and the second substrate, to achieve uniformity of the thickness of the assembled display substrate assembly everywhere, so as to improve quality of a picture displayed in the display apparatus.