The invention discloses a tissue culture propagation method for obtaining a cibotium barometz green globular body. The method includes the steps of obtaining an aseptic explant; inducing the green globular body, wherein the green globular body is obtained after being cultivated in two inducing pre-culture media and then cultivated in a green globular body inducing culture medium; cultivating the green globular body in two propagation culture media alternately, and conducting differentiation culture and rooting culture to obtain a tissue culture seedling. By means of the method, the rare and endangered fern type cibotium barometz green globular body is successfully induced, the propagation efficiency is high, the inducing rate of the green globular body reaches 90-94%, the propagation cycle is short, the method has great significance in increasing the population quantity of rare and endangered fern type cibotium barometz and relieving the endangered situation, and meanwhile the in-vitro conservation of germplasm resources of endangered species is achieved.