The invention relates to an automotive interior energy-storage fluorescent paint and a preparation method thereof. The fluorescent paint comprises, by weight, 40-50 parts of thermoplastic acrylic resin, 5-15 parts of chlorinated polypropylene resin, 10-20 parts of fluorescent powder, 1-5 parts of abrasive powder, 0.1-0.5 parts of anti-sediment agent, 0.2-0.5 part of flatting agent, 0.5-1.5 parts of antioxidant and 15-25 parts of mixed solvent. The preparation method includes sequentially adding mixed solvent, thermoplastic acrylic resin, chlorinated polypropylene resin, anti-sediment agent and abrasive powder to an agitator vessel, agitating and mixing fully to obtain a mixture, transferring the mixture to a horizontal type grinder, grinding the mixture fully until fineness thereof is not larger than 20 micrometers, and then transferring the ground mixture to the agitator vessel, adding flatting agent, antioxidant and florescent powder according to part by weight and stirring and then mixing completely so as to obtain the automotive interior energy-storage fluorescent paint. Compared with the prior art, the fluorescent paint has the advantages of high adhesion, high light intensity, long lighting time, high stability, wear resistance and the like, can emit light at night when applied to automotive interiors, and is energy-saving, environmental friendly, convenient and practical.