The invention provides a testing method for an influence on operation safety of a tunnel in service by electromechanical facility shedding. The method comprises: a, constructing a virtual environment of automobile driving in a tunnel under the condition of electromechanical facility shedding; b, collecting behavior information of a driver carrying out simulated driving in the virtual environment; and c, carrying out an analysis and evaluation on the collected information and determining safety of the electromechanical facility shedding in the tunnel. On the basis of the virtual reality technology and the eye tracker structural principle, a virtual reality experiment platform that is used for testing the influence on driving safety by the electromechanical facility shedding accident in the running tunnel is established, so that direct testing on the electromechanical facility shedding accident in the actual running tunnel does not needed. Moreover, the efficiencies of electromechanical facility shedding grading of the running tunnel as well as driving safety influence evaluation can be improved; quantitative testing of influences on functionality and safety of the tunnel operation by different electromechanical facility shedding accidents in a virtual operation tunnel scene can be realized; a novel high-efficiency and energy-saving method is provided for electromechanical facility shedding grading evaluation of the running tunnel; the lots of manpower and material costs are substantially reduced; and the testing accuracy is improved.