The invention relates to an indoor region location optimization method and system. The method comprises acquiring signal intensity indicator values of a plurality of sensor moving nodes to be located in a communication range; assembling the acquired unique identifiers of the moving nodes, the signal intensity indicator values, a unique identifier of a home gateway node and the current time into a data packet, and transmitting to a positioning server; the positioning server smoothing the signal intensity values according to the received data packet; performing positive logic judgment based on the intensity values after smoothing to obtain a possible regional position list of the moving nodes; performing anti-logic judgment based on the intensity values after smoothing to obtain a impossible regional position list of the moving nodes; removing impossible regions from the possible regional list to obtain a final regional list; and through a reliability calculation method, selecting the regional position with the highest credibility in the regional list as the final locating position. By introducing mixed logic, the method can be used for indoor region location and improving the accuracy of indoor region location.