The invention relates to a traditional Chinese medicine composition capable of enriching and nourishing blood, a preparation method and application thereof. The active component of the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 20 parts of Chinese angelica, 1 to 8 parts of white peony root, 7 to 18 parts of prepared rehmannia root, 1 to 20 parts of longan pulp, 5 to 15 parts of Chinese yam, 1 to 13 parts of radix codonopsis, 3 to 12 parts of wolfberry, 12 to 20 parts of spine date seed, 2 to 10 parts of orange peel, 1 to 5 parts of villous amomum fruit, 3 to 9 parts of donkey-hide gelatin, 1 to 6 parts of jujube, and 1 to 5 parts of licorice. The provided traditional Chinese medicine composition can relieve the symptoms caused by blood deficiency, has a good taste, does not have any toxic or side effect, is safe, and is easy to promote.