In order to solve the problems of cell apoptosis, mutation, cell damage, and neural stem cell differentiation of neural stem cells, long operating time, uneven sizes of cell globes and the like which are caused in the passage process of neural stem cells, the invention provides a stem cell globe cutting and collecting device. The stem cell globe cutting and collecting device comprises a stem cell globe cutter, a stem cell globe collecting bottle, and an air pump provided with positive-pressure and negative-pressure two-way air extracting functions, wherein stem cell globe cutter is connected with the stem cell globe collecting bottle through a pipeline, the stem cell globe cutter is provided with a pipe, and two sieves arranged at an interval are fixed in the pipe. With the adoption of the device, and a stem cell globe cutting and separating passage method, the technical problem of large possibilities of differentiation and death existing in the in-vitro culture of the neural stem cells is solved, the purposes of long-term culture and amplification of the human neural stem cells are achieved, and in addition, the properties of the human neural stem cells are stable, and the quality of the human neural stem cells is controllable.