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发明公开
CN105177188A 同时检测多种辣椒病毒的四重RT-PCR检测引物及方法 无效 - 驳回

同时检测多种辣椒病毒的四重RT-PCR检测引物及方法
摘要：
本发明公开了一种同时检测多种辣椒病毒的四重RT-PCR检测引物及方法，本方法采用如SEQ ID NO：1-SEQ ID NO：8所示的四对引物，一次RT-PCR可同时检测辣椒4种病毒：蚕豆萎蔫病毒2号(Broad bean wilt virus 2，BBWV 2)、辣椒叶脉黄化病毒(Pepper vein yellows virus，PeVYV)、辣椒叶脉斑驳病毒(Chilli veinal mottle virus，ChiVMV)以及辣椒轻斑驳病毒(Pepper mild mottle virus，PMMoV)。与目前单重RT-PCR和双重RT-PCR检测方法相比，本发明方法用一个RT-PCR可同时检测辣椒4种病毒，检测的病毒种类增多，检测效率大为提高，检测成本显著降低。
摘要（英）：

The invention discloses quadruple RT-PCR (reverse transcription-polymerase chain reaction) detection primers and a method capable of simultaneously detecting multiple chili viruses. The method adopts four pairs of primers disclosed as SEQ ID NO:1-SEQ ID NO:8; and one RT-PCR process can simultaneously detect four chili viruses: Broad bean wilt virus 2 (BBWV 2), Pepper vein yellows virus (PeVYV), Chilli veinal mottle virus (ChiVMV) and Pepper mildmottle virus (PMMoV). Compared with the existing single RT-PCR and duplex RT-PCR detection methods, the method disclosed by the invention can simultaneously detect the four chili viruses by one RT-PCR process, and is capable of detecting more virus varieties, greatly enhancing the detection efficiency and obviously lowering the detection cost.

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