The invention provides a water locating-plugging integration method for a horizontal well. The method comprises the following steps: 1, a well deviation angle is larger than 45 degrees, a horizontal well section adopts coupling chamfering oil tubes, and all switches are turned on; 2, the pressure is applied from the oil tubes, the pressure is transferred to the oil tubes under a pump through a rod pump supporting seat, a rubber barrel of a controllable cup packer is released and opened under the action of hydraulic pressure, and seat sealing is realized; 3, a wellhead is sampled, the type and the content of a tracer agent in output liquid are analyzed, and a water drainage section is determined; 4, a casing pipe is filled with water from the wellhead, then the pressure is applied according to a command; pressure wave is transferred into an inlet end oil tube of an oil well pump through a check valve, and a switch of the water drainage section is automatically turned off after receiving the command. When plugging is needed again, the operation is repeated on the ground and closed step by step. A locating-plugging water tube pillar is composed of the packer, a device for placing the tracer agents with different models, a water control switch and the like. Each section of production layer adopts the packer to be separated, the water drainage section can be judged according to the tracer agent in the output liquid, the switch of the corresponding section is turned off, and thus the purpose of locating-plugging integration is realized.