The invention discloses graphene eye movement piece comprising a flexible substrate, a graphene nano wall arranged on the flexible substrate, and a first electrode and a second electrode which are arranged on the graphene nano wall. The invention also discloses an eye movement psychological adjustment instrument comprising an I/O converter, a lower computer, a CPLD module, an upper computer, an LED lamp, a voice metronome and the graphene eye movement piece, wherein the lower computer is connected with the I/O converter, the CPLD module, the upper computer, the LED lamp and the voice metronome respectively; the CPLD module is connected with the first electrode of the graphene eye movement piece; the I/O converter is connected with the second electrode of the graphene eye movement piece. The invention further discloses an eye movement testing method. The invention can provide two excitation modes of photo-stimulation and metronome, and can provide timely analysis of eye movement reactions.