The invention discloses sesame oil taking liquorice glycoside as an antioxidant. The sesame oil is characterized by being prepared through adding liquorice glycoside in the sesame oil. The sesame oil has the following advantages: the natural food sweetening agent namely liquorice glycoside is applied to the sesame oil, and as proved by an experiment, liquorice glycoside also has antioxidation like the chemical synthesis antioxidant TBHQ, can prolong the storage life of the sesame oil, is harmless to a human body, and can improve the sesame oil quality.