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发明公开
CN105200035A 高GC含量大片段DNA的体外组装方法及应用 有权 转让

高GC含量大片段DNA的体外组装方法及应用
摘要：
本发明涉及一种DNA体外组装方法，具体地，本发明涉及高GC含量大片段DNA的体外组装方法及应用。更具体地，本发明人利用改进的Gibson组装方法，在体外成功拼接了来自始旋链霉菌的原始霉素II生物合成基因簇，其可适用于高GC含量DNA大片段组装，并可以明显提高组装效率并简化组装过程。
摘要（英）：

The invention relates to an in-vitro assembling method for DNA, in particular to an in-vitro assembling method for high-GC-content large-fragment DNA and application. More specifically, an improved Gibson assembling method is utilized, a prisztinamicin II biosynthesis gene cluster coming from streptomyces pristinaespiralis is successfully spliced in an in-vitro mode, the method can be suitable for assembling the high-GC-content large-fragment DNA, the assembling efficiency can be improved obviously, and the assembling process can be simplified.

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