A display substrate, a display panel cutting method and a display device, which relate to the technical field of display. The display substrate comprises at least two sub-display regions (11) and at least one isolation unit (12). Each isolation unit (12) comprises two stripped isolation patterns (121, 122) disposed on two sides of an adhesive cutting line (13) on the display substrate. The two stripped isolation patterns (121, 122) are respectively located on peripheral regions (111) of two adjacent sub-display regions (11).