The invention discloses a culture medium for culturing dendrobium at home and a preparation method. The culture medium are arranged in five layers, a first layer of medium, a second layer of medium, a third layer of medium, a fourth layer of medium and a fifth layer of medium are arranged from the top to the bottom, pine bark is used as the first layer of medium, half-decomposed pine needles are used as the second layer of medium, pine needles are used as the third layer of medium, pine bark is used as the fourth layer of medium, and small stones are used as the fifth layer of medium. The five layers of mediums are respectively disinfected and laid, different mediums are separated, on the premise that nutrients needed by growth of the dendrobium are provided, smooth water flow, fertilizer maintenance, ventilation, moisture retention and insulation can be achieved, and the survival rate of the dendrobium at home can be greatly raised. A user can enjoy the process while his home becomes better because of cultured fresh dendrobium branches, flowers and plants, the idea that modern people wants to pursue rurality is satisfied, so the culture medium for culturing dendrobium at home and the preparation method has very broad market prospect.