The invention discloses a preparation method of a material with tobacco fragrance. The preparation method is characterized by sequentially comprising the following steps: 1, drying and pulverizing tobacco raw materials, then conducting extraction through an ethanol solution, conducting solid-liquid separation, then conducting decompression condensation on filter liquor, and obtaining tobacco extract A; adding a bio-enzyme preparation to obtained filter residues for conducting enzymatic hydrolysis, conducting solid-liquid separation, then conducting decompression condensation on filter liquor, and obtaining tobacco extract B; mixing the exact B, amino compounds and polyalcohol, conducting heating, making the mixture react, and obtaining a tobacco reagent C; 4, mixing the tobacco exact A and the tobacco reagent C in a certain proportion, and obtaining the material with tobacco fragrance. The preparation method of the material with tobacco fragrance is applicable to different varieties of tobacco products, and real, abundant and rich original tobacco fragrance can be supplied to the tobacco products.