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发明授权
CN105219768B 大豆转化事件SHZD32-01及其检测方法 有权

大豆转化事件SHZD32-01及其检测方法
摘要：
本发明公开了一种包含大豆转化事件SHZD32‑01的大豆植株和种子以及该大豆转化事件SHZD32‑01中独特的DNA分子，本发明也提出了该特异DNA分子的检测方法；所述大豆转化事件SHZD32‑01含有包括下列核酸序列中的至少1个核酸分子：SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ ID NO:4、SEQ ID NO:9及其完全互补序列；所述检测方法包括如下步骤：1)混合样品包括DNA与引物；2)进行核酸扩增反应，产生扩增产物；3)检测扩增产物，该扩增产物包含SEQ ID NO.1或SEQ ID NO.2。本发明的大豆株系含有SHZD32‑01大豆转化事件,表现出很强的草甘膦耐受性，有助于对杂草的控制，大豆转化事件SHZD32‑01的DNA检测对于鉴定样品中的大豆转化事件SHZD32‑01并应用于含有该DNA的大豆育种具有非常价值。
摘要（英）：

The invention discloses a glycine max plant containing a glycine max transformation event SHZD32-01 and a unique DNA molecule in the glycine max transformation event SHZD32-01 and further provides a detection method for the unique DNA molecule. The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. The glycine max plant containing the glycine max transformation event SHZD32-01 shows great glyphosate tolerance, and is beneficial to control over weeds; and the DNA detection of the glycine max transformation event SHZD32-01 has a high value for identifying the glycine max transformation event SHZD32-01 in the sample and breeding of the glycine max containing the DNA.

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