The invention discloses a rapid regeneration tissue culture method for tartary buckwheat. The method comprises the steps of tartary buckwheat aseptic seedling culture, tartary buckwheat multiple shoot induction, tartary buckwheat regeneration bud rooting, and seedling hardening and transplanting, and finally a complete tartary buckwheat regeneration plant is cultured. The rapid regeneration tissue culture method has the advantages of being high in adaptability, easy and convenient to operate, rapid, economical and the like and is the efficient regeneration technology for rapidly propagation of the high-quality tartary buckwheat germplasm. The totipotency of plant tissue cells is mainly utilized, apical meristems of tartary buckwheat are used as an explant, tartary buckwheat multiple shoots are directly induced to be generated and rooted by induction culture of a specific culture medium and adjusting and controlling the strength of environment factors, and then rapid regeneration of tartary buckwheat can be completed.