The invention relates to deer penis liquor and a preparation method thereof. The deer penis wine is prepared by taking deer penises, pilose antlers, deer blood and baijiu as main materials and taking lucid ganoderma, epimedium, Chinese wolfberries, liquorice and schisandra chinensis as auxiliary materials. The deer penis liquor is prepared according to the modern biotechnology, is transparent clear and brownish red, mellow and strong, has long-lasting aftertaste, contains rich nutrient substances, and has high nutrition value and a good health care function. In the deer penis wine, the protein content is 7.2mg/100ml, the total sugar content is 6.00mg/100ml, the reducing sugar content is 5.91mg/100ml, the fat content is 40mg/100ml, and the content of ascorbic acid is 0.565mg/100ml.