The invention relates to a preparation method of paraffin sections, and especially relates to a preparation method of Euchiloglanis kishinouyei Kimu-ra skin paraffin sections. The preparation method comprises steps of sample fixing, dehydration and transparent treatment, paraffin dipping and embedding, slicing and float slice treatment, and dyeing and section sealing, wherein after sample fixing, dehydration treatment is carried out after 1.5 to 2.5h of washing with running water, and dehydration and embedding time is shortened to 12.5h. According to the preparation method, running water washing is adopted, so that the preparation method is convenient, cost is reduced, and the time from dehydration to embedding is shorter, Euchiloglanis kishinouyei Kimu-ra skin tissue can be completely cut off in a shorter time, a problem that skin tissue is fragile is solved, and it is beneficial for large scale paraffin section preparation and study on Euchiloglanis kishinouyei Kimu-ra skin structures.