The invention relates to biological macromolecular detection and specifically relates to electrophoretic analysis detection. A micro-channel electrophoretic quantitative analysis device comprises an electrophoretic detection system; the electrophoretic detection system comprises a mixture to be analyzed, a micro channel, and a fluorescence detection device, the mixture to be analyzed comprises a standard reference and a component to be analyzed; the micro channel is provided with a positive terminal and a negative terminal; the diameter of cross section of the micro channel is in the millimeter level, the micro channel is full of electrolyte; the mixture to be analyzed is arranged in the negative terminal, a detection area is arranged in the micro channel, and the fluorescence detection device faces the detection area. According to the technical scheme, the structure of micro-channel polymer electrophoretic system is optimized; and the micro channel can separate and analyze the biological macromolecular substances. The micro channel can be used to separate the mixture to be analyzed. Electrolyte can separate the mixture to be analyzed according to the different molecular weights during the electrophoresis process.