The invention discloses a culture method for improving yield of cistanche and a combined fertilizer used for cistanche culture, which belong to the technical field of traditional Chinese medicine culture. The culture method comprises the following steps: (1) culturing or selecting a hosted plant, (2) ditching around the root part of the hosted plant and sowing cistanche seeds in a ditch; the method is characterized by comprising fertilizing soil at the root part of the hosted plant before, when or/and after the cistanche is inoculated, and the fertilizing is performed by applying a plurality of following fertilizers simultaneously or respectively in proportion by weight: 500 to 1800kg/mu of animal manure, 5 to 30kg/mu of potassium sulphate compound fertilizer, 0 to 30kg/mu of urea, 0 to 200kg of dried cynanchum komarovii and 0.5 to 2.5kg of green muscardine fungus conidial powder; an organic fertilizer is 20-30% in water content and is prepared from materials of chicken manure, sheep manure, cow dung and/or camel manure. The culture method can effectively promote growth of the hosted plant, and improve hosting point number and yield of the cistanche relative to a contrast by fertilization schedule improvement.