The invention discloses a disposable infusion apparatus assembling and winding-bundling all-in-one machine and a use method. The all-in-one machine comprises a catheter feeding mechanism, an regulator assembling mechanism, an adjusting roller assembling mechanism, a big plastic needle gluing mechanism, a big plastic needle assembling mechanism, a vein needle gluing mechanism, a vein needle assembling mechanism, an infusion apparatus winding mechanism, an infusion apparatus bundling mechanism and a catheter conveying mechanism. A production line corresponding to the all-in-one machine can avoid the problem of production resource waste, and also can increase the product yield, the sterile control cost is lowered, the number of operators is reduced, the production cost is lowered, and the production speed is increased.