The invention discloses an overcurrent protection circuit with adjustable intermittent time. The overcurrent protection circuit comprises a current detection unit, a reference voltage unit, a comparison unit, a delay trigger unit and a master control chip unit, wherein output ends of the current detection unit and the reference voltage unit are connected with an input end of the comparison unit; the output end of the comparison unit is connected with the input end of the delay trigger unit; and the output end of the delay trigger unit is connected with the input end of the master control chip unit. According to the overcurrent protection circuit, overcurrent and short-circuit protection functions of a PWM control chip are expanded; the overcurrent protection circuit can be applied to a plurality of switching power circuits, such as a single-ended circuit, a symmetric circuit and the like, with topological structures; the overcurrent protection circuit is convenient to adjust, safe and reliable; the power loss in an overcurrent protection process of a power supply is effectively reduced; and the reliability of a switching power supply is improved.