The invention provides a positive and negative sequence separation method for self-adaptive compensation on DC deviation of a double-fed wind power generation set. The positive and negative sequence separation method comprises the following steps of acquiring a stator three-phase voltage, a stator three-phase current and a rotor three-phase current and carrying out 3S/2S conversion; determining a stator voltage deviation signal, a stator current deviation signal and a rotor current deviation signal through an integral closed-loop control; and acquiring a synchronous stator voltage, a synchronous stator current and a synchronous rotor current, and carrying out 2S/3S conversion on the synchronous stator voltage, the synchronous stator current and the synchronous rotor current. The positive and negative sequence separation method for self-adaptive compensation on the DC deviation of the double-fed wind power generation set, provided by the invention, has higher suppress ability on a low-frequency signal, and the influence of reducing the stability of a frequency locking ring and phase locking accuracy due to the existing DC deviation is eliminated.