Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same. Provided herein are stable multianalyte quality control materials for monitoring the performance of various diagnostic testing methodologies in clinical laboratories. The presently described multianalyte control materials address the problem of unstable control solutions, can quickly and easily be prepared at the point of use, and can meet the quality control needs of a lab with regards to the stability of analytes.