The invention provides a plastic particle spraying equipment of glass substrates, wherein plastic particles are used for supporting glass substrates of LCD. The plastic particle spraying equipment comprises a box body, a workbench, a transferring mechanism, a water film moulding mechanism, a spraying mechanism, and a control device. The plastic particle spraying equipment is provided with the water film moulding mechanism, so that liquid water can be sprayed onto the lower end surfaces of the glass substrates via the water film moulding meahcnism, obtain liquid water film between the glass substrates and the workbench, when the glass substrates are separated from the workbench, conductive migration of the plastic particles on the glass substrates caused by static electricity can be prevented by the liquid water film, and dispersion uniformity of the plastic particles on the glass substrates is guaranteed. The invention also provides a plastic particle spraying method.