The invention discloses a cultivating method of loach larvae. The cultivating method comprises the following steps: (1) preparing a loach pond: applying a fermentation organic fertilizer or biological fertilizer to an inner pond; (2) cultivating algae bloom loach larvae: feeding egg yolk, wheel animalcule and ground water earthworm, and controlling the oxygen aeration of pond water; and (3) cultivating sepia loach larvae: transferring the sepia loach larvae cultivated in the inner pond to gullies of an orchard to cultivate, feeding a powdery loach feed with the protein content of 40%-50% and water earthworms, and feeding an expanded particle feed with the particle size of 1.0mm when the loach larvae grow to be inched loach larvae. According to the cultivating method, the bottom material and the water quality of the pond are improved, the biological structure of natural forages is improved, the forages are scientifically fed, toxic and harmful substances such as ammonia nitrogen, nitrite and the like in the pond are controlled, dissolved oxygen of pond water is increased, and the natural forages of planktons are increased, so that the inched loach larvae have strong physiques and are high in survival rate, diseases of the loach larvae are reduced, and the rapid growth of the loach larvae is promoted.