The present invention discloses a nano-scale biological fiber whitening mask and a use method thereof, wherein the nano-scale biological fiber whitening mask comprises a nano-scale biological fiber membrane and a whitening nanometer emulsion. According to the present invention, the fine fibers having the diameter of about 10-50 nm on the nano-scale biological fiber membrane are used, wherein the fine fibers are finer than the skin groove and can deepen into the skin groove so as to increase the contact area of the nanometer emulsion and the skin surface; and the effects of whitening component protection and strong penetrating into the skin of the nanometer emulsion are combined, such that the biological utilization efficiency of the function component is improved so as to well provide the whitening effect within the short time.