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发明公开
CN105296646A 猪源性成分鉴定及其制品中多物种种源成分的检测试剂盒 有权

猪源性成分鉴定及其制品中多物种种源成分的检测试剂盒
摘要：
本发明提供了猪源性成分鉴定及其制品中多物种种源成分的检测试剂盒，属于动物种源性成分分子检测技术领域。本发明试剂盒包括SEQ ID No.2&3所示引物、反应试剂以及阳性和空白对照。此外还包括狐、水貂、鼠、鸡、鸭和马属的特异性引物，其核苷酸序列如SEQ ID No.4～15所示。采用本发明的引物扩增样品DNA，对PCR扩增产物进行分析，可判定样品中是否含有猪源性成分，以及是否掺有马属、狐、水貂、鼠、鸡、鸭等其他动物成分。本发明为肉类、皮张和饲料中上述动物源性成分的分子鉴定及其中掺假成分的检测提供了有效、精准、可靠的手段，具有简便、快速、全面、特异性强的特点，且成本较低，适用性广。
摘要（英）：

Belonging to the technical field of animal origin component molecular detection, the invention provides a detection kit for pig origin component identification and detection of multi-species origin components in products. The kit includes primers shown as SEQ ID No.2&3, a reaction reagent, positive control and blank control. In addition, the kit also includes specific primers of fox, mink, rat, chicken, duck and equus, and the nucleotide sequences thereof are shown as SEQ ID No.4-25. The primers involved in the invention are employed for amplification of the sample DNA and analysis of the PCR amplification product to determine whether the sample contains pig origin components and is mixed with equus, fox, mink, rat, chicken, duck and other animal components. The kit provided by the invention provides an effective, accurate and reliable means for animal origin component molecular identification and detection of adulterated components therein, and has the characteristics of simplicity, fastness, comprehensiveness, strong specificity, low cost, and wide applicability.

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