利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN105296653B 采用荧光RT-PCR技术检测周氏啮小蜂Hsp83基因表达的方法 失效 - 权利终止

采用荧光RT-PCR技术检测周氏啮小蜂Hsp83基因表达的方法
摘要：
本发明公开了一种采用荧光RT‑PCR技术检测周氏啮小蜂Hsp83相对表达量的方法。本发明中周氏啮小蜂Hsp83实时荧光RT‑PCR检测方法的建立，为研究周氏啮小蜂Hsp83表达调控机理及其生态防治奠定基础。研究温度的变化对于周氏啮小蜂体内Hsp83基因表达量的影响，研究结果可为科学利用天敌昆虫周氏啮小蜂提供理论基础，这对于以后在何种室外温度下放飞周氏啮小蜂防治美国白蛾具有重要意义。本发明为在mRNA水平对Hsp83基因的相对定量分析提供了技术平台。
摘要（英）：

The invention discloses a method for detecting the relative expression quantity of Chouioia cunea Yang Hsp83 by adopting the fluorescent RT-PCR technique. In the invention, the establishment of the real-time fluorescent RT-PCR detection method of Chouioia cunea Yang Hsp83 lays the foundation for researches on the expression regulation and control mechanism of Chouioia cunea Yang Hsp83 and ecological prevention and control of Chouioia cunea Yang; the influence of temperature variation on the expression quantity of Chouioia cunea Yang in vivo Hsp83 gene is further studied, so that the research result can provide a theoretical basis for scientific utilization of natural enemy insects Chouioia cunea Yang, and further has an important significance to the research that under what outdoor temperature can Chouioia cunea Yang be let to fly to achieve prevention and control of hyphantria cunea. The method further provides a technical platform for relatively quantitative analysis of Hsp83 gene at the mRNA level.

公开/授权文献
0/0