The invention discloses a method for propagating moghania macrophylla by tissue culture, and the method comprises the following steps: (1) taking and disinfecting explants; (2) gaining aseptic test-tube plantlets; (3) carrying out multiplication culture on the test-tube plantlets; (4) carrying out strong plantlet culture on the test-tube plantlets; (5) carrying out rooting culture on the test-tube plantlets; and (6) carrying out acclimatization and transplant on the test-tube plantlets. According to the method, stems with buds and defoliated stem tips are taken as explants, and through propagation by tissue culture, the culture cycle can be shortened, and high-quality plantlets of moghania macrophylla can be quickly propagated. The bud propagation coefficient of plantlets after being cultured for 30 days can reach 7.0 times, and through propagation expanding, strong plantlet culture and rooting culture, the rooting rate of tissue culture plantlets is 92.5-95.5%, and the survival rate of plantlets transplanted in media is 90.3-96.5%, so that the method is capable of effectively solving the large-scale plantlet raising problem of moghania macrophylla.