The invention discloses soybean oil containing stevia rebaudiana glycoside natural anti-oxidant and having sweet taste. The soybean oil is characterized by being prepared by adding stevia rebaudiana glycoside to a soybean oil body. The soybean oil has the advantages that a natural food sweetening agent, namely the stevia rebaudiana glycoside, is applied to the soybean oil, and it is proved through tests that the anti-oxidation effect of the stevia rebaudiana glycoside is the same as that of antioxidant, namely tert-butylhydroquinone (TBHQ), which is obtained through chemical synthesis, the shelf life of the soybean oil can be prolonged, no harm is caused to the human body, and quality of the soybean oil can be further improved.