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Invention Grant
CN105316421B 用于检测与肺癌相关的SHOX2基因启动子区甲基化程度的试剂盒 有权

用于检测与肺癌相关的SHOX2基因启动子区甲基化程度的试剂盒
Abstract:
本发明公开了一种用于检测与肺癌相关的SHOX2基因启动子甲基化程度的试剂盒，其中包含目的基因引物对和内参基因引物对；以及检测目的基因和内参基因的Taqman探针，和用作阻断作用的阻断剂。所述目的基因引物对包括序列如SEQ ID NO.1所示的目的基因正向引物和序列如SEQ ID NO.2所示的目的基因反向引物；所述内参基因引物对包括序列如SEQ ID NO.3所示的内参基因正向引物和序列如SEQ ID NO.4所示的内参基因反向引物。所述检测的目的基因Taqman探针如SEQ ID NO.5所示，内参基因Taqman探针如SEQ ID NO.6所示。阻断剂序列如SEQ ID NO.7和SEQ ID NO.8所示。
Abstract(English):

The invention discloses a Kit for detecting the methylation level of a lung cancer associated SHOX2 gene promoter region, which comprises a target gene primer pair, a reference gene primer pair, Taqman probes, and blocking agents, wherein the Taqman probe is used for detecting target genes and reference genes, and the blocking agent has a blocking effect. The target gene primer pair comprises a target gene forward primer shown in a sequence SEQ ID NO.1 and a target gene reverse primer shown in a sequence SEQ ID NO.2; and the reference gene primer pair comprises a reference gene forward primer shown in a sequence SEQ ID NO.3 and a reference gene reverse primer shown in a sequence SEQ ID NO.4. The target gene Taqman probe for detecting is shown in a sequence SEQ ID NO.5, and the reference gene Taqman probe is shown in a sequence SEQ ID NO.6. The blocking agents are shown in a sequence SEQ ID NO.7 and a sequence SEQ ID NO.8.

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