The invention relates to an ultra-high resolution imaging method used for a rapid moving object, comprising: according to the position of an ultra-high resolution imaging target on a moving object sample, setting an ROI including the ultra-high resolution imaging target; obtaining the moving speed of the ultra-high resolution imaging target and the moving speed and position of the ROI, and obtaining the imaging frame frequency of an ultra-high resolution imaging module; calculating the size of an imaging shooting area Sp according to the relation between the imaging frame frequency and the Sp; defining a pre-visualization area Si including the ultra-high resolution imaging target on the sample, wherein the Si has the same size with the Sp, and the same moving speed with the ROI; adjusting the position of the Si or the Sp according to the motion of the ROI so as to keep the Si and the Sp overlapped; and according to the Si, performing ultra-high resolution imaging on the ultra-high resolution imaging target on the moving object sample. The ultra-high resolution imaging method can eliminate the influence of object motion to ultra-high resolution imaging.