The invention discloses an electrospun fiber experimental sample collecting device, which has the advantages that the structure is simple; the design is ingenious; the layout is reasonable. By aiming at the problems that the traditional electrospun fiber collecting device needs to be operated under different parameters (the distance between an electrospun spray head and the voltage applied onto a collecting plate), so that a large amount of work is needed, the workload is great, the work efficiency is low, and the like, the invention provides the electrospun fiber experimental sample collecting device which can automatically complete the electrospun fiber collection under a plurality of groups of different voltage conditions and different height difference conditions; the workload of operators is greatly reduced; the work efficiency is improved; in addition, the manufacturing process is simple; the manufacturing cost is low, so that the electrospun fiber experimental sample collecting device has various advantages. The electrospun fiber experimental sample collecting device is particularly suitable for being popularized and applied in the field, and the market prospect is very wide.