The invention discloses an intelligent integrated testing device and method of a low-temperature insulated gas cylinder valve. The intelligent integrated testing device comprises an industrial control computer (1), wherein the industrial control computer is connected with a PLC control system (2) through an RS232 serial communication interface, the input end of the PLC control system is connected with automatic/manual switching button and limit switch signals of a stop valve service life test unit (3), a shell pressure-resisting test unit (4) and a valve action performance test unit (5), the output end of the PLC control system is connected with control solenoid valve coils of the stop valve service life test unit, the shell pressure-resisting test unit and the valve action performance test unit through a contactless relay (6), and pressure sensor signals of the stop valve service life test unit, the shell pressure-resisting test unit and the valve action performance test unit are connected with the PLC control system through an A/D module (7). The intelligent integrated testing device integrates various testing devices of the valve and achieves automatic testing control through the industrial control computer and the PLC control system.