The invention discloses a multilayer fertilization machine used for autumn ploughing. The machine is characterized by comprising a frame (2) which is provided with at least a fertilizer box (5) and a layering fertilization mechanism, wherein the layering fertilization mechanism has the following concrete structure: the mechanism comprises at least a support (12); one end of the support (12) is fixedly arranged on the frame (2); the other end of the support (12) is provided with a ditching shovel (9) and a vertically-arranged fertilizer guide groove (10); the ditching shovel (9) is arranged at the front of the fertilizer guide groove (10); a fertilizer delivery pipe is arranged between the fertilizer guide groove (10) and the fertilizer box (5); the fertilizer guide groove (10) is a tube-shaped body; the interior of the tube-shaped body is provided with spiral blades; the surface of the tube-shaped body is provided with a fertilizer discharging outlet (14); and a fertilizer guide plate (11) is arranged at the place of the fertilizer discharging outlet (14) and extends outwards along the tube-shaped body. Compared with the prior art, the multilayer fertilization machine provided by the invention has the characteristics of simple structure, good layering fertilization effect, significant fertilization layers, capability of improving fertilization utilization rate, etc.