The present invention relates to an enema preparation for treatment of diabetic nephropathy, which can effectively solve the drug problem in diabetic nephropathy, the enema preparation comprises the following raw materials in parts by weight: 5-20 parts of Chinese angelica, 5-20 parts of white peony root, 5-15 parts of ligusticum wallichii, 5-20 parts of Poria, 5-25 parts of white atractylodes rhizome, 5-15 parts of alisma, 5-15 parts of betel nut, 5-15 parts of habenaria stenopetala, 10-50 parts of shepherd's purse, 5-30 parts of Chinese rhubarb, 5-15 parts of semen lepidii and 5-15 parts of fructus liquidambaris, the enema preparation can effectively improve the integral of clinical symptom and microalbumin excretion rate of patients with diabetic nephropathy, delays the progression of the course of disease of the diabetic nephropathy, and is an innovation on the drug for treatment of diabetic nephropathy.